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Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction to produce a stable triazole linkage. It also reacts with cyclooctynes via a copper-free βclick chemistryβ reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This reaction is bioorthogonal or in other words compatible with biological systems in that itβ²s components do not react with the biological environment. This reagent is also know as Biotin-Azide.
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